Site-specific recombinases: tools for genome engineering

Trends Genet. 1993 Dec;9(12):413-21. doi: 10.1016/0168-9525(93)90104-p.


Site-specific recombinases from bacteriophage and yeasts have been developed as novel tools for manipulating DNA both in the test-tube and in living organisms. We discuss the characteristics of these enzyme systems, review their application in genetic and developmental studies and speculate on their future potential for large-scale directed modifications of eukaryotic genomes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Base Sequence
  • DNA Nucleotidyltransferases / metabolism*
  • Genetic Engineering*
  • Genome*
  • Molecular Sequence Data
  • Substrate Specificity


  • DNA Nucleotidyltransferases