Cloning and expression of a human glutamate transporter

Biochem Biophys Res Commun. 1994 Feb 28;199(1):171-6. doi: 10.1006/bbrc.1994.1210.

Abstract

A complementary DNA clone was isolated from human brain that encodes a glutamate transporter. Injection of RNA transcribed from this cDNA into Xenopus oocytes resulted in expression of a transport activity with a high affinity for glutamate (Km = 78.4 microM) and a dependency on external Na+. The cDNA sequence predicts a protein of 542 amino acids that is highly homologous to the congeneric proteins from rat and mouse brains. RNA blotting analyses revealed the expression both in the brain and in peripheral tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acid Transport System X-AG
  • Animals
  • Base Sequence
  • Biological Transport / drug effects
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Gene Expression
  • Genes
  • Glycoproteins / drug effects
  • Glycoproteins / genetics*
  • Humans
  • Membrane Glycoproteins / genetics
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • Tissue Distribution
  • Xenopus laevis

Substances

  • Amino Acid Transport System X-AG
  • DNA, Complementary
  • Glycoproteins
  • Membrane Glycoproteins
  • RNA, Messenger

Associated data

  • GENBANK/D26443