Vasculotropin/vascular endothelial growth factor induces differentiation in cultured osteoblasts

Biochem Biophys Res Commun. 1994 Feb 28;199(1):380-6. doi: 10.1006/bbrc.1994.1240.

Abstract

Vasculotropin/vascular endothelial growth factor (VAS/VEGF) is an angiogenic growth factor whose biological activity seems to be restricted in vitro to vascular endothelial cells. We describe here that fetal bovine osteoblasts (OB) bind VAS/VEGF but do not proliferate upon its addition. However VAS/VEGF induces migration, PTH-dependent cAMP accumulation and alkaline phosphatase increase when added to OB. The maximal effects reach levels comparable to that obtained with bone morphogenetic protein 2 (BMP-2), although the VAS/VEGF concentrations required are at least 100 fold lower. Our results suggest that VAS/VEGF could be an important regulator of osteoblastic differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Cell Differentiation / drug effects
  • Cell Movement / drug effects
  • Endothelial Growth Factors / pharmacology*
  • Fibroblast Growth Factor 2 / pharmacology
  • Growth Substances / pharmacology*
  • Humans
  • Lymphokines / pharmacology*
  • Osteoblasts / cytology*
  • Osteoblasts / enzymology
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors

Substances

  • Endothelial Growth Factors
  • Growth Substances
  • Lymphokines
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors
  • Fibroblast Growth Factor 2
  • Alkaline Phosphatase