Abstract
The Opaque-2 (O2) gene from maize encodes a transcriptional activator of the b-ZIP class. We have isolated and characterized a gene from sorghum, related in sequence to the O2 gene from maize. A single copy of the gene is present in sorghum. Both genomic and cDNA sequences of the O2-related sorghum gene were determined. The sequence is highly homologous to maize O2 both in the promoter and in the coding region. The most closely related sequences contain the b-ZIP domain with only 11 amino acid substitutions in a total of 122 residues. In transient expression assays, the sorghum O2-related coding sequence, expressed from a CaMV 35S promoter, activates expression from the maize b-32 promoter as effectively as that obtained with the maize O2 sequence.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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DNA-Binding Proteins / genetics*
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G-Box Binding Factors
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Genes, Plant*
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Glucuronidase / biosynthesis
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Glucuronidase / genetics
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Leucine Zippers / genetics
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Open Reading Frames
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Plant Proteins / genetics*
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Poaceae / genetics*
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Promoter Regions, Genetic*
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Protein Sorting Signals / genetics
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Transcription Factors / genetics*
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Transfection
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Zea mays / genetics*
Substances
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DNA-Binding Proteins
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G-Box Binding Factors
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Oligodeoxyribonucleotides
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Plant Proteins
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Protein Sorting Signals
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Transcription Factors
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opaque-2 protein, Zea mays
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Glucuronidase