Effect of aspirin on incidence and growth of aberrant crypt foci induced in the rat colon by 1,2-dimethylhydrazine

Cancer Lett. 1994 Jan 15;76(1):5-9. doi: 10.1016/0304-3835(94)90127-9.

Abstract

The aim of this work was to investigate if the possible chemopreventive effect of aspirin (ASA) on rat colon carcinogenesis could be detected with a medium-term assay. The end-point chosen was the inhibition of incidence and growth of putative preneoplastic lesions, the aberrant crypt foci (ACF) induced in the rat colon by two administrations of 1,2-dimethylhydrazine (DMH, 25 mg/kg p.o.). At both 4 and 8 weeks after the starting of the carcinogenic treatment the incidence of total ACF was reduced of 60% in rats receiving ASA (10 mg/kg/day p.o.) for 12 consecutive days during the initiation treatment with DMH. Also the number of the larger foci (with 3 or more crypts) was significantly lower in ASA-treated rats at both time-points (about 70% reduction). Moreover, concomitant ASA treatment determined a significant decrease of the mean number of crypts per focus at week 8. These results indicate that the chemopreventive effect of ASA on chemically-induced rat colon carcinogenesis observed in long-term studies may be detected in this relatively short assay.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1,2-Dimethylhydrazine
  • Animals
  • Anticarcinogenic Agents / therapeutic use*
  • Aspirin / therapeutic use*
  • Body Weight / drug effects
  • Carcinogens*
  • Cell Division / drug effects
  • Colon / drug effects*
  • Colon / pathology
  • Colonic Neoplasms / chemically induced
  • Colonic Neoplasms / pathology
  • Colonic Neoplasms / prevention & control*
  • Dimethylhydrazines*
  • Drug Interactions
  • Male
  • Precancerous Conditions / chemically induced
  • Precancerous Conditions / pathology
  • Precancerous Conditions / prevention & control*
  • Rats
  • Rats, Sprague-Dawley
  • Time Factors

Substances

  • Anticarcinogenic Agents
  • Carcinogens
  • Dimethylhydrazines
  • 1,2-Dimethylhydrazine
  • Aspirin