Pericentrin, a highly conserved centrosome protein involved in microtubule organization

Cell. 1994 Feb 25;76(4):639-50. doi: 10.1016/0092-8674(94)90504-5.


Antisera from scleroderma patients that react widely with centrosomes in plants and animals were used to isolate cDNAs encoding a novel centrosomal protein. The nucleotide sequence is consistent with a 7 kb mRNA and contains an open reading frame encoding a protein with a putative large coiled-coil domain flanked by noncoiled ends. Antisera recognize a 220 kd protein and stain centrosomes and acentriolar microtubule-organizing centers, where the protein is localized to the pericentriolar material (hence, the name pericentrin). Anti-pericentrin antibodies disrupt mitotic and meiotic divisions in vivo and block microtubule aster formation in Xenopus extracts, but do not block gamma-tubulin assembly or microtubule nucleation from mature centrosomes. These results suggest that pericentrin is a conserved integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens / physiology*
  • Autoantigens / chemistry*
  • Centrioles / ultrastructure
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Fluorescent Antibody Technique
  • Gene Expression
  • Humans
  • Microscopy, Electron
  • Microtubule-Associated Proteins / chemistry*
  • Microtubules / chemistry*
  • Molecular Sequence Data
  • Molecular Weight
  • RNA, Messenger / genetics
  • Restriction Mapping
  • Spindle Apparatus / ultrastructure
  • Tubulin / metabolism


  • Antigens
  • Autoantigens
  • DNA, Complementary
  • Microtubule-Associated Proteins
  • RNA, Messenger
  • Tubulin
  • pericentrin

Associated data

  • GENBANK/U05823