Mouse JunD negatively regulates fibroblast growth and antagonizes transformation by ras

Cell. 1994 Feb 25;76(4):747-60. doi: 10.1016/0092-8674(94)90513-4.


As NIH 3T3 fibroblasts become quiescent, the level of c-Jun protein decreases while JunD accumulates. When resting cells are stimulated with fresh serum, nuclear-localized JunD is rapidly degraded, followed by resynthesis of both c-Jun and JunD later in G1. Overexpression of JunD results in slower growth and an increase in the percentage of cells in G0/G1 while c-Jun overexpression produces larger S/G2 and M phase populations. In addition, JunD partially suppresses transformation by an activated ras gene whereas c-Jun cooperates with ras to transform cells. These data indicate that two closely related transcription factors can function in an opposing manner.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Cycle*
  • Cell Transformation, Neoplastic / genetics*
  • Fibroblasts / cytology
  • Genes, ras*
  • Mice
  • Molecular Sequence Data
  • Peptides / chemistry
  • Proto-Oncogene Proteins c-jun / genetics*
  • Proto-Oncogene Proteins c-jun / metabolism


  • Peptides
  • Proto-Oncogene Proteins c-jun