Two tyrosinase nonapeptides recognized on HLA-A2 melanomas by autologous cytolytic T lymphocytes

Eur J Immunol. 1994 Mar;24(3):759-64. doi: 10.1002/eji.1830240340.

Abstract

A number of cytolytic T lymphocyte (CTL) clones derived from several melanoma patients have been found to recognize a majority of melanomas from HLA-A2 patients. We have reported previously that two such CTL clones recognize a product of the tyrosinase gene that is presented by HLA-A2. Here we show that one of these CTL clones recognizes a peptide encoded by the first nine amino acids of the putative signal sequence of tyrosinase. The other CTL clone recognizes a different tyrosinase peptide corresponding to amino acids 368-376. Both peptides contain consensus motifs of HLA-A2 binding peptides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cytotoxicity, Immunologic
  • DNA Primers / chemistry
  • HLA-A2 Antigen / immunology
  • Humans
  • In Vitro Techniques
  • Melanoma / immunology*
  • Molecular Sequence Data
  • Monophenol Monooxygenase / genetics
  • Monophenol Monooxygenase / immunology*
  • Peptides / immunology*
  • Recombinant Proteins
  • T-Lymphocytes, Cytotoxic / immunology*
  • Tumor Cells, Cultured

Substances

  • DNA Primers
  • HLA-A2 Antigen
  • Peptides
  • Recombinant Proteins
  • Monophenol Monooxygenase