Cloning of cmpE, a plasmid-borne catechol 2,3-dioxygenase-encoding gene from the aromatic- and chloroaromatic-degrading Pseudomonas sp. HV3

Gene. 1994 Jan 28;138(1-2):119-21. doi: 10.1016/0378-1119(94)90792-7.

Abstract

Pseudomonas sp. strain HV3 degrades aromatics and chloroaromatics. It harbours a mega-plasmid, designated pSKY4, from which the gene cmpE, encoding a catechol 2,3-dioxygenase (C23O) catalyzing the conversion of catechol to 2-hydroxymuconic semialdehyde, was cloned and sequenced. The deduced amino acid (aa) sequence shows the highest homology, 52%, to the deduced aa sequences of xylE1 and dmpB. The deduced 307-aa sequence of cmpE contains the extradiol ring-cleavage signature in the same position as other 307-aa C23O-encoding genes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Catechol 2,3-Dioxygenase
  • Cloning, Molecular / methods
  • Dioxygenases*
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Oxygenases / biosynthesis*
  • Oxygenases / genetics*
  • Plasmids
  • Pseudomonas / enzymology
  • Pseudomonas / genetics*
  • Pseudomonas / metabolism
  • Restriction Mapping
  • Sequence Homology, Amino Acid

Substances

  • Oxygenases
  • Dioxygenases
  • Catechol 2,3-Dioxygenase

Associated data

  • GENBANK/J01766
  • GENBANK/L10655
  • GENBANK/M17159
  • GENBANK/M33263
  • GENBANK/M65205
  • GENBANK/X06412
  • GENBANK/X60740