Abstract
Pseudomonas sp. strain HV3 degrades aromatics and chloroaromatics. It harbours a mega-plasmid, designated pSKY4, from which the gene cmpE, encoding a catechol 2,3-dioxygenase (C23O) catalyzing the conversion of catechol to 2-hydroxymuconic semialdehyde, was cloned and sequenced. The deduced amino acid (aa) sequence shows the highest homology, 52%, to the deduced aa sequences of xylE1 and dmpB. The deduced 307-aa sequence of cmpE contains the extradiol ring-cleavage signature in the same position as other 307-aa C23O-encoding genes.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Catechol 2,3-Dioxygenase
-
Cloning, Molecular / methods
-
Dioxygenases*
-
Genes, Bacterial*
-
Molecular Sequence Data
-
Oxygenases / biosynthesis*
-
Oxygenases / genetics*
-
Plasmids
-
Pseudomonas / enzymology
-
Pseudomonas / genetics*
-
Pseudomonas / metabolism
-
Restriction Mapping
-
Sequence Homology, Amino Acid
Substances
-
Oxygenases
-
Dioxygenases
-
Catechol 2,3-Dioxygenase
Associated data
-
GENBANK/J01766
-
GENBANK/L10655
-
GENBANK/M17159
-
GENBANK/M33263
-
GENBANK/M65205
-
GENBANK/X06412
-
GENBANK/X60740