Light and heavy chains specifying a human IgM kappa autoantibody to a T-cell receptor V beta-antigen

Immunol Lett. 1993 Nov;38(3):223-7. doi: 10.1016/0165-2478(93)90010-y.

Abstract

Humans frequently produce serum IgM autoantibodies reactive with T-cell receptor beta chains at a determinant defined by peptides corresponding to the first complementarity determining region. It is likely that this determinant serves as a public idiotype involved in immunoregulation. Following screening of culture supernatants from over 60 Epstein-Barr virus-carrying B-cell lines of normal and neoplastic origin, we identified a line, IARC307, that secretes an IgM kappa protein showing marked specificity for the V beta 8.1 CDR1 sequence CKPISGHNSLFQWYRQT. We cloned and sequenced the complete variable regions of the V kappa and VH chains used by the autoantibody. The light chain has a V kappa III sequence related to the 'a' subgroup and uses J kappa 2. The heavy chain has a VHIII sequence essentially identical to the germ-line sequence DP54 and uses the JH6C minigene. The CDR3 is unique, differing from those of other autoantibodies. The antibody is rigorously specific in its specificity for the V beta 8 peptide and does not show polyspecificity for protein or DNA antigens.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Autoantibodies / chemistry*
  • Base Sequence
  • DNA, Complementary / biosynthesis
  • Humans
  • Immunoglobulin Heavy Chains / chemistry*
  • Immunoglobulin M / chemistry*
  • Immunoglobulin Variable Region
  • Immunoglobulin kappa-Chains / chemistry*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Messenger / isolation & purification
  • Receptors, Antigen, T-Cell, alpha-beta / immunology*

Substances

  • Autoantibodies
  • DNA, Complementary
  • Immunoglobulin Heavy Chains
  • Immunoglobulin M
  • Immunoglobulin Variable Region
  • Immunoglobulin kappa-Chains
  • RNA, Messenger
  • Receptors, Antigen, T-Cell, alpha-beta