Reduction of collagen degradation in experimental granulation tissue by vitamin E and selenium

J Clin Periodontol. 1994 Jan;21(1):45-7. doi: 10.1111/j.1600-051x.1994.tb00275.x.


The importance of antioxidants in inflammatory tissues has been studied in vivo. Degradation of homologous 3H-collagen powder by experimental granulation tissue induced by cellulose sponges in the rat was monitored as the radioactivity excreted in urine. By administering pharmacological doses of both vitamin E and selenium subcutaneously and by injection into sponges implanted subcutaneously, this breakdown of collagen was reduced. Injections in the sponges also arrested the maturation of the granulation tissue. Vitamin E and selenium are potential inhibitors of the free oxygen radicals from phagocytic inflammatory cells. It is therefore suggested that these radicals may play a rôle in the collagen destruction by granulation tissues, as in periodontitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Collagen / drug effects*
  • Collagen / metabolism*
  • Collagen / urine
  • Edema / pathology
  • Extracellular Matrix / pathology
  • Granulation Tissue / drug effects*
  • Granulation Tissue / metabolism*
  • Granulation Tissue / pathology
  • Injections, Subcutaneous
  • Male
  • Neutrophils / pathology
  • Prostheses and Implants
  • Rats
  • Rats, Sprague-Dawley
  • Selenium / administration & dosage
  • Selenium / pharmacology*
  • Skin / drug effects
  • Skin / metabolism
  • Skin / pathology
  • Surgical Sponges
  • Tritium
  • Vitamin E / administration & dosage
  • Vitamin E / pharmacology*


  • Tritium
  • Vitamin E
  • Collagen
  • Selenium