A plasma lipoprotein containing only apolipoprotein E and with gamma mobility on electrophoresis releases cholesterol from cells

Proc Natl Acad Sci U S A. 1994 Mar 1;91(5):1834-8. doi: 10.1073/pnas.91.5.1834.


Previous studies have identified lipid-poor high density lipoproteins with electrophoretic pre-beta mobility as the initial acceptors of cell-derived cholesterol in human plasma. These lipoproteins contain apolipoprotein A-I (apo A-I) as their sole apolipoprotein. In the present study, incubation of human plasma with [3H]cholesterol-laden skin fibroblasts has led to the identification of another lipoprotein that serves as a potent initial acceptor of cell-derived cholesterol. This lipoprotein, which we term gamma-LpE, exhibits gamma mobility on agarose gel electrophoresis. As determined by nondenaturing PAGE and by electron microscopy, the size of the spherical particle ranges between 12 and 16 nm. SDS/PAGE and subsequent immunoblotting identified apoE as its sole apolipoprotein. Plasma from normal and apoA-I-deficient mice, but not from apoE-deficient mice, released [3H]cholesterol from fibroblasts into a gamma-migrating lipoprotein. Cell culture media from hepatoma cells or mouse peritoneal macrophages, both of which contain apoE of cellular origin, also promoted efflux of [3H]cholesterol from fibroblasts into a gamma-migrating fraction. This was not observed with cell culture medium from fibroblasts alone. In conclusion, our results strongly indicate the presence in human plasma of a lipoprotein containing only apoE, gamma-LpE, which is secreted by peripheral cells and is a potent acceptor of cell-derived cholesterol.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Apolipoprotein A-I / deficiency
  • Apolipoproteins E / deficiency
  • Apolipoproteins E / isolation & purification
  • Apolipoproteins E / metabolism*
  • Biological Transport, Active
  • Blood Protein Electrophoresis
  • Cells, Cultured
  • Cholesterol / metabolism*
  • Fibroblasts / metabolism
  • Humans
  • In Vitro Techniques
  • Lipoproteins / blood*
  • Lipoproteins / isolation & purification
  • Lipoproteins / metabolism
  • Mice


  • Apolipoprotein A-I
  • Apolipoproteins E
  • Lipoproteins
  • Cholesterol