Leucine synthesis in spinach chloroplasts: partial characterization of 2-isopropylmalate synthase

Biol Chem Hoppe Seyler. 1993 Dec;374(12):1105-8. doi: 10.1515/bchm3.1993.374.7-12.1105.


Leucine synthesis was found to be localized in chloroplasts of higher plants. The introductory enzyme of the isopropylmalate pathway, the 2-isopropylmalate synthase, was strongly feedback-regulated by leucine. The pH optimum of the enzyme was between 7.0 and 9.0. The Km values were 5 microM for acetyl-CoA and 75 microM for 2-oxoisovalerate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2-Isopropylmalate Synthase / metabolism*
  • Chloroplasts / enzymology
  • Chloroplasts / metabolism*
  • Hydrogen-Ion Concentration
  • Leucine / biosynthesis*
  • Plants / enzymology
  • Plants / metabolism*


  • 2-Isopropylmalate Synthase
  • Leucine