Low single channel conductance of the major skeletal muscle chloride channel, ClC-1

Biophys J. 1994 Jan;66(1):149-52. doi: 10.1016/S0006-3495(94)80753-2.


We expressed the skeletal muscle chloride channel, ClC-1, in HEK293 cells and investigated it with the patch-clamp technique. Macroscopic properties are similar to those obtained after expression in Xenopus oocytes, except that faster gating kinetics are observed in mammalian cells. Nonstationary noise analysis revealed that both rat and human ClC-1 have a low single channel conductance of about 1 pS. This finding may explain the lack of single-channel data for chloride channels from skeletal muscle despite its high macroscopic chloride conductance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Chloride Channels / biosynthesis
  • Chloride Channels / physiology*
  • Electrophysiology / methods
  • Embryo, Mammalian
  • Embryo, Nonmammalian
  • Female
  • Humans
  • Kidney
  • Membrane Potentials
  • Muscle Proteins / biosynthesis
  • Muscle Proteins / physiology*
  • Muscles / physiology*
  • Oocytes / physiology
  • Rats
  • Transfection
  • Xenopus


  • CLC-1 channel
  • Chloride Channels
  • Muscle Proteins