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Comparative Study
, 40 (3), 411-6

Retinol, Alpha-Tocopherol, Lutein/Zeaxanthin, Beta-Cryptoxanthin, Lycopene, Alpha-Carotene, Trans-Beta-Carotene, and Four Retinyl Esters in Serum Determined Simultaneously by Reversed-Phase HPLC With Multiwavelength Detection

Affiliations
  • PMID: 8131277
Comparative Study

Retinol, Alpha-Tocopherol, Lutein/Zeaxanthin, Beta-Cryptoxanthin, Lycopene, Alpha-Carotene, Trans-Beta-Carotene, and Four Retinyl Esters in Serum Determined Simultaneously by Reversed-Phase HPLC With Multiwavelength Detection

A L Sowell et al. Clin Chem.

Abstract

We describe the use of HPLC with multiwavelength detection to measure retinol, alpha-tocopherol, lutein/zeaxanthin, beta-cryptoxanthin, lycopene, alpha-carotene, trans-beta-carotene, beta-carotene, and the linoleate, oleate, palmitate, and stearate esters of retinol in a single 200-microL serum sample. The method is sensitive enough to detect individual retinyl esters in fasting serum from a nonhyperlipidemic population and requires only 12 min for each sample. Serum concentration ranges and means are reported for retinol, alpha-tocopherol, lutein/zeaxanthin, beta-cryptoxanthin, lycopene, alpha-carotene, trans-beta-carotene, and the sum of the retinyl esters from serum analyses of 3480 participants from several different studies.

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