Inactivation of the streptokinase gene prevents Streptococcus equisimilis H46A from acquiring cell-associated plasmin activity in the presence of plasminogen

FEMS Microbiol Lett. 1994 Feb 1;116(1):107-12. doi: 10.1111/j.1574-6968.1994.tb06683.x.


The streptokinase gene of Streptococcus equisimilis H46 was inactivated by plasmid insertion mutagenesis to study the relationship between elaboration of streptokinase and acquisition of cell-associated plasmin activity after incubation of wild-type and mutant cells in media containing plasminogen or plasmin. The results showed that H46A binds both the zymogen and active enzyme, generates surface-associated plasmin activity in the presence of plasminogen when producing streptokinase, and expresses its plasmin(ogen) receptor(s) independently of a functional streptokinase gene. At least part of the plasmin(ogen) binding capacity may be due to the glyceraldehyde-3-phosphate dehydrogenase type of receptor molecule, as judged by the detection of the corresponding gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA, Bacterial
  • Fibrinolysin / metabolism*
  • Humans
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Plasminogen / metabolism*
  • Receptors, Cell Surface / metabolism
  • Receptors, Urokinase Plasminogen Activator
  • Restriction Mapping
  • Streptococcal Infections / microbiology
  • Streptococcus / enzymology*
  • Streptococcus / genetics
  • Streptokinase / genetics
  • Streptokinase / metabolism*


  • DNA, Bacterial
  • PLAUR protein, human
  • Receptors, Cell Surface
  • Receptors, Urokinase Plasminogen Activator
  • Plasminogen
  • Streptokinase
  • Fibrinolysin