The protein phosphatase calcineurin is essential for NaCl tolerance of Saccharomyces cerevisiae

J Biol Chem. 1994 Mar 25;269(12):8792-6.


NaCl-sensitive yeast mutants were isolated to identify genes essential for NaCl tolerance. Complementation of a mutant highly sensitive to Na+ and Li+ led to the isolation of the CNB1 gene. This gene encodes the regulatory subunit (CNB) of the Ca2+/calmodulin-dependent protein phosphatase calcineurin. Cells deficient in CNB accumulated Li+ due to reduced expression of ENA1, a gene encoding a P-type ATPase involved in Na+ and Li+ efflux. In addition, the K+ transport system of cnb1 delta cells was not converted to the high affinity state that facilitates better discrimination of K+ over Na+. Thus the cnb1 delta strain resembled a trk1 mutant. These results indicate that adaptation to NaCl stress in Saccharomyces cerevisiae requires a signal transduction pathway involving Ca2+ and protein phosphorylation-dephosphorylation. In this pathway, calcineurin would coordinate gene expression and activity of ion transporters to facilitate ion homeostasis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / genetics
  • Base Sequence
  • Biological Transport, Active
  • Calcineurin
  • Calmodulin-Binding Proteins / physiology*
  • Cation Transport Proteins*
  • Chromosome Mapping
  • Cloning, Molecular
  • Fungal Proteins / genetics
  • Genes, Fungal
  • Genetic Complementation Test
  • Homeostasis
  • Lithium Chloride
  • Molecular Sequence Data
  • Phosphoprotein Phosphatases / physiology*
  • Restriction Mapping
  • Saccharomyces cerevisiae / physiology*
  • Saccharomyces cerevisiae Proteins*
  • Sodium / metabolism*
  • Sodium Chloride / metabolism
  • Sodium-Potassium-Exchanging ATPase
  • Water-Electrolyte Balance


  • Calmodulin-Binding Proteins
  • Cation Transport Proteins
  • ENA1 protein, S cerevisiae
  • Fungal Proteins
  • Saccharomyces cerevisiae Proteins
  • Sodium Chloride
  • Sodium
  • Calcineurin
  • Phosphoprotein Phosphatases
  • Adenosine Triphosphatases
  • Sodium-Potassium-Exchanging ATPase
  • Lithium Chloride

Associated data

  • GENBANK/Z26521