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. 1994 Mar;124(6):883-92.
doi: 10.1083/jcb.124.6.883.

En bloc incorporation of coatomer subunits during the assembly of COP-coated vesicles

S Hara-Kuge  1 , O Kuge, L Orci, M Amherdt, M Ravazzola, F T Wieland, J E Rothman
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Free PMC article

En bloc incorporation of coatomer subunits during the assembly of COP-coated vesicles

S Hara-Kuge et al. J Cell Biol. 1994 Mar.
Free PMC article

Erratum in

  • J Cell Biol 1994 Jul;126(2):589

Abstract

The cDNA encoding epsilon-COP, the 36-kD subunit of coatomer, was cloned from a bovine liver cDNA library and sequenced. Immunoblotting with an anti-epsilon-COP antibody showed that epsilon-COP exists in COP-coated vesicles as well as in the cytosolic coatomer. Using the cloned cDNA, recombinant His6- tagged epsilon-COP was overexpressed in cultured Chinese hamster ovary (CHO) cells, from which metabolically radiolabeled coatomer was purified by taking advantage of the His6 tag. Radiolabeled coatomer was employed to establish that all the subunits of the coatomer enter coated vesicles as an intact unit.

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