Induction of immune unresponsiveness to concordant islet xenografts by intrahepatic preimmunization and transient immunosuppression

J Clin Invest. 1994 Mar;93(3):1312-4. doi: 10.1172/JCI117088.


Streptozotocin-induced, diabetic mice (C57BL/6) were preimmunized by injecting 25 low temperature, cultured Wistar-Furth (WF) rat islets into the portal vein, and the recipients received one injection of mouse and rat antilymphocyte sera. 3 wk later, fresh WF islets were transplanted under the kidney capsule of the preimmunized recipients, and normoglycemia was maintained in all 13 recipients for 60 d. Removal of the grafts at 60 d returned the mice to a diabetic state. Transplants of fresh WF islets under the kidney capsule without pretreatment of the recipients had a mean survival time of 16.5 +/- 2.5 d. These findings demonstrate that immune unresponsiveness can be achieved across a concordant, islet xenograft barrier within 3 wk after intrahepatic preimmunization with a small number of donor rat islets and transient immunosuppression with antilymphocyte sera.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antilymphocyte Serum / therapeutic use*
  • Graft Survival
  • Immunization
  • Islets of Langerhans / immunology
  • Islets of Langerhans Transplantation / immunology*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Rats
  • Rats, Inbred WF
  • Transplantation, Heterologous / immunology*


  • Antilymphocyte Serum