Cellular distribution of human leucocyte adhesion molecule ICAM-3

J Clin Pathol. 1994 Feb;47(2):143-7. doi: 10.1136/jcp.47.2.143.

Abstract

Aims: To describe the distribution of the recently cloned human leucocyte adhesion molecule ICAM-3 in normal and neoplastic tissues and cell lines.

Methods: A panel of four monoclonal antibodies to ICAM-3 were used to stain cell lines and sections of human lymphoid tissues using the alkaline phosphatase-anti-alkaline phosphatase immunocytochemical method (APAAP).

Results: In peripheral blood ICAM-3 was detected on monocytes, granulocytes, and most lymphocytes. In sections of human lymphoid tissue the antigen was also found on most lymphocytes, but many of the proliferating B cells found in the germinal centres of secondary lymphoid follicles were ICAM-3 negative. ICAM-3 was also found on neoplastic white cells (in chronic lymphocytic leukaemia, hairy cell leukaemia, acute and chronic myeloid leukaemia, and multiple myeloma) with the exception of Reed-Sternberg cells in Hodgkin's disease, many of which were negative. ICAM-3 was consistently absent from cells and tissues of non-haemopoietic origin. Endothelium (which expresses ICAM-1) was negative for ICAM-3, with the exception of vessels in some neoplastic lymphoid samples which showed variable staining for ICAM-3.

Conclusions: These findings suggest that ICAM-3 is essentially restricted to the haemopoietic system and is reciprocal in its expression to ICAM-1, in that it is present on resting cells and its level falls as a result of cell activation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Antigens, CD*
  • Antigens, Differentiation*
  • Cell Adhesion Molecules / analysis*
  • Cell Adhesion Molecules / blood
  • Granulocytes / chemistry
  • Humans
  • Immunoenzyme Techniques
  • Leukemia / metabolism
  • Lymphocytes / chemistry
  • Lymphoma / metabolism
  • Monocytes / chemistry
  • Neoplasm Proteins / analysis
  • Tumor Cells, Cultured

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, Differentiation
  • Cell Adhesion Molecules
  • ICAM3 protein, human
  • Neoplasm Proteins