Abstract
YY1 is a zinc finger transcription factor whose DNA-binding motif exhibits the properties of an initiator element. Only three factors were required to direct specific basal transcription on a supercoiled template DNA carrying the YY1 initiator: YY1, general transcription factor IIB, and RNA polymerase II. This minimal in vitro reaction did not require the TATA-binding protein (TBP). We propose that, under appropriate conditions, YY1 can function like TBP, as a factor that binds to the core promoter and recruits the polymerase to the initiation complex.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Adenoviridae / genetics
-
Base Sequence
-
DNA, Superhelical / genetics*
-
DNA, Viral / genetics
-
DNA-Binding Proteins / physiology*
-
Dependovirus / genetics
-
Erythroid-Specific DNA-Binding Factors
-
HeLa Cells
-
Humans
-
Molecular Sequence Data
-
Promoter Regions, Genetic / physiology
-
RNA Polymerase II / physiology*
-
TATA-Box Binding Protein
-
Templates, Genetic
-
Transcription Factor TFIIB
-
Transcription Factors / physiology*
-
Transcription, Genetic / physiology*
-
YY1 Transcription Factor
Substances
-
DNA, Superhelical
-
DNA, Viral
-
DNA-Binding Proteins
-
Erythroid-Specific DNA-Binding Factors
-
TATA-Box Binding Protein
-
Transcription Factor TFIIB
-
Transcription Factors
-
YY1 Transcription Factor
-
YY1 protein, human
-
RNA Polymerase II