TATA-binding protein-independent initiation: YY1, TFIIB, and RNA polymerase II direct basal transcription on supercoiled template DNA

Cell. 1994 Mar 25;76(6):1115-21. doi: 10.1016/0092-8674(94)90387-5.

Abstract

YY1 is a zinc finger transcription factor whose DNA-binding motif exhibits the properties of an initiator element. Only three factors were required to direct specific basal transcription on a supercoiled template DNA carrying the YY1 initiator: YY1, general transcription factor IIB, and RNA polymerase II. This minimal in vitro reaction did not require the TATA-binding protein (TBP). We propose that, under appropriate conditions, YY1 can function like TBP, as a factor that binds to the core promoter and recruits the polymerase to the initiation complex.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviridae / genetics
  • Base Sequence
  • DNA, Superhelical / genetics*
  • DNA, Viral / genetics
  • DNA-Binding Proteins / physiology*
  • Dependovirus / genetics
  • Erythroid-Specific DNA-Binding Factors
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • Promoter Regions, Genetic / physiology
  • RNA Polymerase II / physiology*
  • TATA-Box Binding Protein
  • Templates, Genetic
  • Transcription Factor TFIIB
  • Transcription Factors / physiology*
  • Transcription, Genetic / physiology*
  • YY1 Transcription Factor

Substances

  • DNA, Superhelical
  • DNA, Viral
  • DNA-Binding Proteins
  • Erythroid-Specific DNA-Binding Factors
  • TATA-Box Binding Protein
  • Transcription Factor TFIIB
  • Transcription Factors
  • YY1 Transcription Factor
  • YY1 protein, human
  • RNA Polymerase II