Agonist-specific coupling of a cloned Drosophila octopamine/tyramine receptor to multiple second messenger systems

EMBO J. 1994 Mar 15;13(6):1325-30.

Abstract

A cloned seven transmembrane-spanning Drosophila octopamine/tyramine receptor, permanently expressed in a Chinese hamster ovary cell line, both inhibits adenylate cyclase activity and leads to the elevation of intracellular Ca2+ levels by separate G-protein-coupled pathways. Agonists of this receptor (octopamine and tyramine), differing by only a single hydroxyl group in their side chain, may be capable of differentially coupling it to different second messenger systems. Thus, a single receptor may have a different pharmacological profile depending on which second messenger system is used to assay its efficacy.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • CHO Cells
  • Calcium / metabolism
  • Cloning, Molecular
  • Cricetinae
  • Cyclic AMP / metabolism
  • Drosophila / metabolism*
  • Octopamine / metabolism*
  • Radioligand Assay
  • Receptors, Biogenic Amine / genetics
  • Receptors, Biogenic Amine / metabolism*
  • Second Messenger Systems*
  • Tyramine / metabolism*

Substances

  • Receptors, Biogenic Amine
  • norsynephrine receptor
  • tyramine receptor
  • Octopamine
  • Cyclic AMP
  • Calcium
  • Tyramine