STF-IL-4: a novel IL-4-induced signal transducing factor

EMBO J. 1994 Mar 15;13(6):1350-6.

Abstract

The mechanism by which interleukin-4 (IL-4) regulates the expression of particular genes is unknown. We have determined that IL-4 induces a DNA binding factor (termed STF-IL-4) which has a strong affinity for an IFN-gamma activation site (GAS). Interestingly, STF-IL-4 also binds to the IL-4 responsive promoter for the Ig heavy chain germline epsilon transcript. The IL-4 dependent activation of STF-IL-4 is rapid, does not require protein synthesis and results in the sequential appearance of binding activity first in the cytoplasm and then later in the nucleus. Activation of STF-IL-4 is sensitive to tyrosine kinase inhibitors and the active factor is tyrosine phosphorylated. This pattern of activation is similar to the activation of interferon-induced transcription factors. STF-IL-4 appears to be a new member of a growing family of cytokine-induced transcriptional regulators.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Biological Transport
  • Cell Nucleus / metabolism
  • Cells, Cultured
  • Cytoplasm / metabolism
  • DNA / metabolism
  • DNA-Binding Proteins / biosynthesis*
  • DNA-Binding Proteins / metabolism
  • Gene Expression Regulation
  • Immunoglobulins / metabolism
  • Interleukin-4 / physiology*
  • Mice
  • Molecular Sequence Data
  • Phosphorylation
  • Promoter Regions, Genetic
  • Signal Transduction*
  • Tyrosine / metabolism

Substances

  • DNA-Binding Proteins
  • Immunoglobulins
  • Interleukin-4
  • Tyrosine
  • DNA