Promoter targeting by adenovirus E1a through interaction with different cellular DNA-binding domains

Nature. 1994 Apr 7;368(6471):520-5. doi: 10.1038/368520a0.


A puzzling property of the transcriptional activators encoded by several animal viruses is their ability to function promiscuously. The adenovirus E1a protein, for example, stimulates transcription of adenoviral genes as well as a wide variety of other viral and cellular genes. We show that E1a can interact with several classes of cellular DNA-binding domains and thereby be recruited to diverse promoters. Our results explain how a single protein can regulate transcription of multiple genes that lack a common promoter element.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Activating Transcription Factor 2
  • Adenovirus E1A Proteins / metabolism*
  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • CHO Cells
  • Conserved Sequence
  • Cricetinae
  • Cyclic AMP Response Element-Binding Protein / metabolism*
  • DNA / metabolism
  • Escherichia coli
  • Gene Expression Regulation, Viral*
  • Glutathione Transferase / genetics
  • Glutathione Transferase / metabolism
  • Molecular Sequence Data
  • Promoter Regions, Genetic*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Transcription Factors / metabolism
  • Transcription, Genetic


  • Activating Transcription Factor 2
  • Adenovirus E1A Proteins
  • Cyclic AMP Response Element-Binding Protein
  • Recombinant Proteins
  • Transcription Factors
  • DNA
  • Glutathione Transferase