Characterization of human uroguanylin: a member of the guanylin peptide family

Am J Physiol. 1994 Feb;266(2 Pt 2):F342-8. doi: 10.1152/ajprenal.1994.266.2.F342.


Guanylin, a peptide homologue of the bacterial heat-stable enterotoxins (ST), is an endogenous activator of guanylate cyclase C (GC-C). We have initiated a search for other members of the guanylin peptide family and in the current study describe a "guanylin-like peptide" from human urine. Bioactivity was monitored by determining the effect of urine extracts on T84 cell guanosine 3',5'-cyclic monophosphate (cGMP) levels. Purification yielded two bioactive peaks of peptides that, when sequenced by NH2-terminal analysis, possessed 15 and 16 amino acids. The sequence of the smaller peptide represented an NH2-terminal truncation of the larger peptide. We have termed the larger peptide human uroguanylin; it has the following amino acid sequence: NDDCELCVNVACTGCL. Human uroguanylin shares amino acid sequence homology with guanylin and ST. Synthetic uroguanylin increased cGMP levels in T84 cells, competed with 125I-labeled ST for receptors, and stimulated Cl- secretion as reflected by an increased short-circuit current. Thus we report the isolation from human urine of a unique peptide, uroguanylin, that behaves in a manner similar to guanylin and appears to be a new member of this peptide family.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Chlorides / metabolism
  • Colon / drug effects
  • Colon / physiology
  • Cyclic GMP / metabolism
  • Escherichia coli
  • Gastrointestinal Hormones*
  • Humans
  • In Vitro Techniques
  • Intestinal Mucosa / drug effects
  • Intestinal Mucosa / physiology
  • Male
  • Mass Spectrometry
  • Molecular Sequence Data
  • Natriuretic Peptides
  • Opossums
  • Peptides / chemistry
  • Peptides / pharmacology
  • Peptides / urine*
  • Radioligand Assay
  • Rats
  • Sequence Homology, Amino Acid


  • Chlorides
  • Gastrointestinal Hormones
  • Natriuretic Peptides
  • Peptides
  • guanylin
  • uroguanylin
  • Cyclic GMP