Cloning and sequencing of an Na+/H+ antiporter gene from the marine bacterium Vibrio alginolyticus

Biochim Biophys Acta. 1994 Mar 23;1190(2):465-8. doi: 10.1016/0005-2736(94)90109-0.


A gene has been cloned from a DNA library from the marine bacterium Vibrio alginolyticus that functionally complements a mutant strain of Escherichia coli, NM81, defective in an Na+/H+ antiporter (NhaA). The cloned Vibrio gene restored NM81 to grow in a medium containing 0.5 M NaCl at pH 7.5 and concomitantly led to an increase in Na+/H+ antiport activity. The nucleotide sequence of the cloned fragment revealed an open reading frame, which encodes a protein with a predicted 383 amino acid sequence and molecular mass of 40,400 Da. The hydropathy profile is characteristic of a membrane protein with 11 membrane spanning regions. The deduced amino acid sequence is 58% identical with E. coli NhaA.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Molecular Sequence Data
  • Sequence Alignment
  • Sodium-Hydrogen Exchangers / genetics*
  • Vibrio / genetics*


  • Sodium-Hydrogen Exchangers

Associated data

  • GENBANK/D25214