Abstract
Tau protein kinase II (TPKII) was reported previously to be composed of a neuron-rich cdc2-related kinase (PSSALRE/cdk5) and 23 kDa subunit. Here we show that the 23 kDa subunit is a putative activator for the kinase activity. Amino acid sequence analysis revealed that the protein was novel and included a partial similarity of amino acids to a cyclin box important for the interaction with cdc2-related kinase. These results suggest that the 23 kDa subunit, but not cyclin, activates cdk5 in neuronal cells, which no longer exhibit cell cycling but are terminally differentiated cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Brain / metabolism
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Cattle
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Cyclin-Dependent Kinase 5
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Cyclin-Dependent Kinases*
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Cyclins / genetics
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DNA Primers / genetics
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DNA Probes / genetics
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DNA, Complementary / genetics
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Enzyme Activation
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Humans
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In Vitro Techniques
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Molecular Sequence Data
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Molecular Weight
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Peptide Fragments / chemistry
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Peptide Fragments / genetics
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Protein Conformation
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Protein Kinases / metabolism*
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Protein Serine-Threonine Kinases / chemistry
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism*
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Sequence Homology, Amino Acid
Substances
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Cyclins
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DNA Primers
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DNA Probes
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DNA, Complementary
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Peptide Fragments
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Protein Kinases
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Cyclin-Dependent Kinase 5
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Protein Serine-Threonine Kinases
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CDK5 protein, human
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Cyclin-Dependent Kinases
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tau protein kinase II
Associated data
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GENBANK/X78883
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GENBANK/X78934