Abstract
A novel cDNA clone was isolated from a yeast Saccharomyces cerevisiae lambda gt11 cDNA library using rabbit anti-rat tropomyosin (TM30nm) polyclonal antibody (RTM8-2). It consists of an open reading frame of 951 bp, encoding 317 amino acid residues. The putative sequence recognized by RTM8-2 was present in Asn-235 to Thr-250. The deduced amino acid sequence and hydropathy plot suggested that this protein has a tropomyosin-homologous sequence, a predicted transmembrane, and a C-terminal basic region. A search of the data bases (EMBL and GenBank) revealed that the 128bp sequence in the 3' untranslated region (3'UTR) is almost identical (96.9%) to the human cDNA clone 54E05 sequence (EMBL accession number Z15978).
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies
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Base Sequence
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Cloning, Molecular
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DNA, Fungal / chemistry
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DNA, Fungal / metabolism*
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Fungal Proteins / biosynthesis*
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Fungal Proteins / chemistry
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Gene Library
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Humans
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Membrane Proteins / biosynthesis*
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Membrane Proteins / chemistry
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Molecular Sequence Data
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Open Reading Frames
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Rabbits / immunology
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Rats
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Restriction Mapping
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Sequence Homology, Amino Acid
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Tropomyosin / biosynthesis*
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Tropomyosin / chemistry
Substances
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Antibodies
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DNA, Fungal
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Fungal Proteins
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Membrane Proteins
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Tropomyosin
Associated data
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GENBANK/X77582
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GENBANK/Z15978