Isolation of a yeast tropomyosin-related cDNA clone that encodes a novel transmembrane protein having a C-terminal highly basic region

Biochem Biophys Res Commun. 1994 Mar 30;199(3):1363-71. doi: 10.1006/bbrc.1994.1381.

Abstract

A novel cDNA clone was isolated from a yeast Saccharomyces cerevisiae lambda gt11 cDNA library using rabbit anti-rat tropomyosin (TM30nm) polyclonal antibody (RTM8-2). It consists of an open reading frame of 951 bp, encoding 317 amino acid residues. The putative sequence recognized by RTM8-2 was present in Asn-235 to Thr-250. The deduced amino acid sequence and hydropathy plot suggested that this protein has a tropomyosin-homologous sequence, a predicted transmembrane, and a C-terminal basic region. A search of the data bases (EMBL and GenBank) revealed that the 128bp sequence in the 3' untranslated region (3'UTR) is almost identical (96.9%) to the human cDNA clone 54E05 sequence (EMBL accession number Z15978).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies
  • Base Sequence
  • Cloning, Molecular
  • DNA, Fungal / chemistry
  • DNA, Fungal / metabolism*
  • Fungal Proteins / biosynthesis*
  • Fungal Proteins / chemistry
  • Gene Library
  • Humans
  • Membrane Proteins / biosynthesis*
  • Membrane Proteins / chemistry
  • Molecular Sequence Data
  • Open Reading Frames
  • Rabbits / immunology
  • Rats
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Sequence Homology, Amino Acid
  • Tropomyosin / biosynthesis*
  • Tropomyosin / chemistry

Substances

  • Antibodies
  • DNA, Fungal
  • Fungal Proteins
  • Membrane Proteins
  • Tropomyosin

Associated data

  • GENBANK/X77582
  • GENBANK/Z15978