Identification of clozapine N(+)-glucuronide in the urine of patients treated with clozapine using electrospray mass spectrometry

Biol Mass Spectrom. 1994 Mar;23(3):147-8. doi: 10.1002/bms.1200230305.


Clozapine N(+)-glucuronide was detected in the urine of five patients chronically treated with clozapine. Identification was made on the basis that the material isolated from urine had the same high-performance liquid chromatographic retention time and positive electrospray mass spectra as that of an authentic reference standard of clozapine N(+)-glucuronide. These results indicate that electrospray mass spectrometry is a valuable technique in the analysis of low-molecular-weight biologically derived N(+)-glucuronide metabolites.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Clozapine / analogs & derivatives*
  • Clozapine / metabolism
  • Clozapine / therapeutic use*
  • Clozapine / urine
  • Glucuronates / urine*
  • Humans
  • Male
  • Mass Spectrometry
  • Middle Aged


  • Glucuronates
  • clozapine N-glucuronide
  • Clozapine