Enhancements to the conjunctival impression cytology technique and examples of applications in a clinico-biochemical study of dry eye

CLAO J. 1994 Jan;20(1):59-63.


The conjunctival impression cytology (CIC) technique was optimized for cell pick-up and resolution of cytologic detail. Use of surfactant-free smaller pore-size Millipore filters yielded consistently high cell pick-up and resolution of cell detail. Staining by the modified Papanicolaou technique in a free-floating format allowed quantitative evaluation of cytologic features. Air exposure of CIC specimens for up to 8 minutes before fixing (as occurs with Nelson's standardized technique) did not adversely affect cell appearances. The optimized method was applied to a group of 50 patients with dry eyes. CIC features were correlated with symptoms, clinical test results, and serum and tear protein levels of dry eye patients.

MeSH terms

  • Conjunctiva / pathology*
  • Cytological Techniques
  • Dry Eye Syndromes / metabolism
  • Dry Eye Syndromes / pathology*
  • Filtration
  • Humans
  • Random Allocation
  • Rose Bengal


  • Rose Bengal