1 kb of the lactase-phlorizin hydrolase promoter directs post-weaning decline and small intestinal-specific expression in transgenic mice

FEBS Lett. 1994 Apr 11;342(3):291-6. doi: 10.1016/0014-5793(94)80519-9.


Adult-type hypolactasia is a genetic condition making approximately one half of the human population intolerant to milk because of abdominal symptoms. The cause is a post-weaning down-regulation of the intestinal-specific enzyme lactase-phlorizin hydrolase (LPH) reducing the intestinal capacity to hydrolyze lactose. We here demonstrate that the stretch -17 to -994 in the pig LPH-promoter carries cis-elements which direct a small intestinal-specific expression and a post-weaning decline of a linked rabbit beta-globin gene. These data demonstrate that the post-weaning decline of LPH is mainly due to a transcriptional down-regulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Animals
  • Gene Expression Regulation*
  • Intestine, Small / enzymology*
  • Lactase-Phlorizin Hydrolase / genetics*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Mice, Transgenic
  • Promoter Regions, Genetic
  • RNA, Messenger / genetics
  • Tissue Distribution
  • Transcription, Genetic


  • RNA, Messenger
  • Lactase-Phlorizin Hydrolase