Cyclin E Controls S Phase Progression and Its Down-Regulation During Drosophila Embryogenesis Is Required for the Arrest of Cell Proliferation

Cell. 1994 Apr 8;77(1):107-20. doi: 10.1016/0092-8674(94)90239-9.

Abstract

Most cells of the dorsal epidermis exit from the mitotic cycle after division 16 in Drosophila embryogenesis. This exit is dependent on the down-regulation of Drosophila cyclin E (DmcycE) during the final mitotic cycle. Ectopic expression of DmcycE after the final mitosis induces entry into S phase and reaccumulation of G2 cyclins and results in progression through a complete additional cell cycle. Conversely, analyses in DmcycE mutant embryos indicate that cyclin E is required for progression through S phase of the mitotic cycle. Moreover, endoreplication, which occurs in late wild-type embryos in the same pattern as DmcycE expression, is not observed in the mutant embryos. Therefore, Drosophila cyclin E, which forms a complex with the Dmcdc2c kinase, controls progression through S phase and its down-regulation limits embryonic proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • CDC2 Protein Kinase / metabolism
  • Cell Cycle*
  • Cyclins / physiology*
  • DNA Primers / chemistry
  • Drosophila melanogaster / cytology
  • Drosophila melanogaster / embryology*
  • Gene Expression Regulation
  • Growth Inhibitors
  • Mitosis
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • S Phase

Substances

  • Cyclins
  • DNA Primers
  • Growth Inhibitors
  • RNA, Messenger
  • CDC2 Protein Kinase