The expression of Vpre-B/lambda 5 surrogate light chain in early bone marrow precursor B cells of normal and B cell-deficient mutant mice

Cell. 1994 Apr 8;77(1):133-43. doi: 10.1016/0092-8674(94)90241-0.

Abstract

Precursor B (pre-B) cells in bone marrow of normal and B cell-deficient mutant mice were analyzed for the expression of Vpre-B/lambda 5 surrogate light chain (SL). The surface expression of SL is confined to the early stages (pro-B and pre-B-I) of pre-B cell development and becomes undetectable once mu heavy chain (microH) is produced. The cell-cycle analysis revealed that cytoplasmic microH+ large cells (large pre-B-II), approximately 30% of which coexpressed SL in the cytoplasm, were most actively cycling, whereas cytoplasmic microH+ small cells (small pre-B-II) were SL- and not in cycle. The analysis of pre-B cells in B cell-deficient mice suggests that the large pre-B-II stage is a critical step for the selection and amplification of cells carrying functionally rearranged microH genes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Surface / metabolism
  • B-Lymphocyte Subsets / physiology
  • B-Lymphocytes / physiology*
  • Bone Marrow Cells*
  • Cell Cycle
  • Cell Differentiation
  • Female
  • Genes, Immunoglobulin*
  • Humans
  • Immunoglobulin Light Chains
  • Immunoglobulin Light Chains, Surrogate
  • Immunoglobulin lambda-Chains / genetics*
  • Immunoglobulin mu-Chains / genetics*
  • Immunoglobulin mu-Chains / metabolism
  • Membrane Glycoproteins / genetics*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Mutant Strains

Substances

  • Antigens, Surface
  • Immunoglobulin Light Chains
  • Immunoglobulin Light Chains, Surrogate
  • Immunoglobulin lambda-Chains
  • Immunoglobulin mu-Chains
  • Membrane Glycoproteins