The Drosophila rolled locus encodes a MAP kinase required in the sevenless signal transduction pathway

EMBO J. 1994 Apr 1;13(7):1628-35.

Abstract

Mitogen-activated protein (MAP) kinases have been proposed to play a critical role in receptor tyrosine kinase (RTK)-mediated signal transduction pathways. Although genetic and biochemical studies of RTK pathways in Caenorhabditis elegans, Drosophila melanogaster and mammals have revealed remarkable similarities, a genetic requirement for MAP kinases in RTK signaling has not been established. During retinal development in Drosophila, the sevenless (Sev) RTK is required for development of the R7 photoreceptor cell. Components of the signal transduction pathway activated by Sev in the R7 precursor include proteins encoded by the gap1, drk, Sos, ras1 and raf loci. In this report we present evidence that a Drosophila MAP kinase, ERK-A, is encoded by the rolled locus and is required downstream of raf in the Sev signal transduction pathway.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calcium-Calmodulin-Dependent Protein Kinases / physiology*
  • DNA Mutational Analysis
  • Drosophila / embryology*
  • Drosophila / genetics
  • Drosophila Proteins*
  • Extracellular Signal-Regulated MAP Kinases*
  • Eye / embryology*
  • Eye / ultrastructure
  • Eye Proteins / physiology*
  • Genetic Complementation Test
  • Membrane Glycoproteins / physiology*
  • Models, Biological
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins c-raf
  • Receptor Protein-Tyrosine Kinases / metabolism
  • Signal Transduction*
  • Suppression, Genetic

Substances

  • Drosophila Proteins
  • Eye Proteins
  • Membrane Glycoproteins
  • Proto-Oncogene Proteins
  • Receptor Protein-Tyrosine Kinases
  • sev protein, Drosophila
  • Proto-Oncogene Proteins c-raf
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Extracellular Signal-Regulated MAP Kinases
  • rl protein, Drosophila