Triticum timopheevii ssp. timopheevii and T. timopheevii ssp. araraticum were analysed by sequential N-banding and genomic in situ hybridization. Three chromosomes, 6At, 1G and 4G, were involved in At-G intergenomic translocations in all six lines analysed. These chromosomes may be derived from a cyclic translocation that is species-specific to T. timopheevii. In contrast, Triticum turgidum has a species-specific cyclic translocation involving chromosomes 4A, 5A and 7B. The discovery of different species-specific chromosome translocations supports the diphyletic hypothesis of the evolution of tetraploid wheats. The results from genomic blocking analysis also revealed that the chromosomes of Aegilops speltoides are closer to the G genome than the B genome chromosomes. The possible role of species-specific translocations in the evolution of wheat is discussed.