Adsorption of aerosil on erythrocyte surface by flow cytometry measurements

Cytometry. 1994 Jan 1;15(1):80-3. doi: 10.1002/cyto.990150113.

Abstract

Light scattering properties of the erythrocytes in less than 0.01% aerosil suspension were measured. It was shown that light scattering properties of intact cells and cells associated with silica (aerosil) are different. It was also established that the aerosil particles are characterized by more intensive autofluorescence in the channel of green fluorescence than the erythrocytes. We propose that this observation allows a facile means of studying the interaction of purified silica and cell membranes. Hemolysis, as a result of interaction between the aerosil particles and the erythrocytes, was confirmed by photometric measurement of hemoglobin in the supernatant.

MeSH terms

  • Adsorption
  • Erythrocyte Membrane / chemistry*
  • Erythrocyte Membrane / drug effects
  • Flow Cytometry*
  • Fluorescence
  • Hemolysis / drug effects
  • Humans
  • Light
  • Scattering, Radiation
  • Silicon Dioxide / chemistry*
  • Silicon Dioxide / pharmacology
  • Surface Properties

Substances

  • Silicon Dioxide