Collagen-binding recombinant fibronectin fragments containing type II domains

FEBS Lett. 1994 Apr 18;343(1):47-50. doi: 10.1016/0014-5793(94)80604-7.


Each of the two type II domains and four larger fragments, containing one or two type II domains of fibronectin, have been expressed in Escherichia coli. A special vector, containing a fragment encoding the cleavage site for Factor Xa, Ile-Glu-Gly-Arg, inserted immediately before the protein fragment of interest, was used. After treatment of the purified fusion proteins with reduced/oxidized glutathione, the correctly folded fibronectin fragments were released by proteolytic digestion with Factor Xa. The largest fragment, consisting of two type II and two type I domains, was the only fragment able to bind to immobilized gelatin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Binding Sites
  • Collagen / metabolism*
  • DNA, Recombinant
  • Escherichia coli / genetics
  • Fibronectins / genetics
  • Fibronectins / metabolism*
  • Molecular Sequence Data
  • Peptide Fragments / genetics
  • Peptide Fragments / metabolism*
  • Recombinant Proteins / metabolism


  • DNA, Recombinant
  • Fibronectins
  • Peptide Fragments
  • Recombinant Proteins
  • Collagen