Abstract
We have cloned the al-2 gene of Neurospora crassa and have analyzed its structure and regulation. The gene encodes a 603-residue polypeptide with a segment homologous to prokaryotic and other eukaryotic phytoene synthases. RNA measurements showed that the level of al-2 mRNA increased over 30-fold in photoinduced mycelia compared with dark-grown mycelia. This observation is consistent with the fact that carotenoid biosynthesis is induced by blue light during growth of N. crassa mycelia. The photoinduced increase in al-2 mRNA levels was not observed in two Neurospora mutants, wc-1 and wc-2, that are defective in all physiological photoresponses.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alkyl and Aryl Transferases*
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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Conserved Sequence
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Gene Expression Regulation, Enzymologic* / radiation effects
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Gene Expression Regulation, Fungal* / radiation effects
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Genes, Fungal*
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Genetic Complementation Test
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Geranylgeranyl-Diphosphate Geranylgeranyltransferase
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Light
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Molecular Sequence Data
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Neurospora crassa / enzymology*
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Neurospora crassa / genetics*
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Promoter Regions, Genetic
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RNA, Messenger / biosynthesis*
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Repetitive Sequences, Nucleic Acid
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Restriction Mapping
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Transcription, Genetic / radiation effects
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Transferases / biosynthesis
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Transferases / genetics*
Substances
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RNA, Messenger
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Transferases
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Alkyl and Aryl Transferases
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Geranylgeranyl-Diphosphate Geranylgeranyltransferase