Increased expression of c-met messenger RNA following acute gastric injury in rats

Biochem Biophys Res Commun. 1994 Apr 15;200(1):536-41. doi: 10.1006/bbrc.1994.1481.


The aim of the present study was to evaluate messenger RNA expression of c-met, a hepatocyte growth factor receptor gene, after gastric mucosal injury in rats. Male Sprague-Dawley rats were fasted for 24 hours, received 0.6 N hydrochloric acid (HCl), and served for polyadenylated RNA extraction from the oxyntic gastric mucosa. The transcripts of rat c-met gene were analyzed by reverse-transcript polymerase chain reaction and Northern blotting. Although it was detected even before the HCl administration, the c-met expression increased 6, 24 and 48 hours after the HCl administration. Thereafter, gastric mucosal injury diminished and the c-met expression declined. Hepatocyte growth factor reportedly plays an important role in gastric cell proliferation. The increased c-met expression indicates that this gene may participate in the healing process of gastric mucosa after injury.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Gastric Mucosa / drug effects
  • Gastric Mucosa / metabolism*
  • Gastric Mucosa / pathology
  • Gene Expression
  • Hepatocyte Growth Factor / metabolism*
  • Hydrochloric Acid / toxicity
  • Male
  • Mice
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Proto-Oncogene Proteins / biosynthesis*
  • Proto-Oncogene Proteins c-met
  • Proto-Oncogenes*
  • RNA, Messenger / biosynthesis*
  • Rats
  • Rats, Sprague-Dawley
  • Receptor Protein-Tyrosine Kinases / biosynthesis*
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic


  • Proto-Oncogene Proteins
  • RNA, Messenger
  • Hepatocyte Growth Factor
  • Proto-Oncogene Proteins c-met
  • Receptor Protein-Tyrosine Kinases
  • Hydrochloric Acid

Associated data

  • GENBANK/S69881