Adrenomedullin stimulates cyclic AMP formation in rat vascular smooth muscle cells

Biochem Biophys Res Commun. 1994 Apr 15;200(1):642-6. doi: 10.1006/bbrc.1994.1496.


A newly identified human peptide, adrenomedullin (AM) increased cAMP levels with dose and time dependencies in a manner similar to that of human calcitonin gene-related peptide (CGRP) in rat vascular smooth muscle cells (VSMC). The EC50 value of human AM is 2 x 10(-8) M which is slightly higher than that of CGRP (8.5 x 10(-9) M). In a receptor binding assay for AM in rat VSMC, the binding of [125I] AM was competitively inhibited by human AM, but not by human CGRP. Thus, AM is thought to increase intracellular cAMP in rat VSMC via its specific receptor to evoke vasodilation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenomedullin
  • Analysis of Variance
  • Animals
  • Antihypertensive Agents / pharmacology*
  • Aorta / drug effects
  • Aorta / metabolism
  • Binding Sites
  • Binding, Competitive
  • Calcitonin Gene-Related Peptide / pharmacology*
  • Cells, Cultured
  • Cyclic AMP / metabolism*
  • Humans
  • Kinetics
  • Male
  • Muscle, Smooth, Vascular / drug effects
  • Muscle, Smooth, Vascular / metabolism*
  • Peptides / metabolism
  • Peptides / pharmacology*
  • Rats
  • Rats, Inbred WKY


  • Antihypertensive Agents
  • Peptides
  • Adrenomedullin
  • Cyclic AMP
  • Calcitonin Gene-Related Peptide