Effect of sanguinarine on the transport of essential nutrients in an everted gut sac model: role of Na+,K(+)-ATPase

Nat Toxins. 1993;1(4):235-40. doi: 10.1002/nt.2620010406.

Abstract

The effect of the argemone alkaloid, sanguinarine, was studied on the active transport of D-glucose and some of the L-amino acids in everted sacs of the small intestine of rats. Sanguinarine (1.0 mumole) was found to inhibit (61%) the transport of D-glucose, while an alkaloid concentration of 0.1 mumole was ineffective. Both 0.1 and 1.0 mumole of sanguinarine had no effect on the transport of the L-amino acids including aspartic acid, lysine, and tyrosine. Sanguinarine showed a dose dependent inhibition of intestinal and hepatic Na+,K(+)-ATPase in a non-competitive manner. The inhibition of Na+,K(+)-ATPase by sanguinarine may in turn inhibit the active transport of D-glucose which requires a sodium pump.

MeSH terms

  • Alkaloids / pharmacokinetics
  • Alkaloids / pharmacology*
  • Amino Acids / metabolism*
  • Animals
  • Benzophenanthridines
  • Biological Transport / drug effects
  • Glucose / metabolism*
  • Intestinal Mucosa / metabolism*
  • Isoquinolines
  • Male
  • Rats
  • Rats, Wistar
  • Sodium-Potassium-Exchanging ATPase / antagonists & inhibitors
  • Sodium-Potassium-Exchanging ATPase / physiology*

Substances

  • Alkaloids
  • Amino Acids
  • Benzophenanthridines
  • Isoquinolines
  • sanguinarine
  • Sodium-Potassium-Exchanging ATPase
  • Glucose