Expression of human nm23-H1 and nm23-H2 proteins in hepatocellular carcinoma

Cancer. 1994 May 1;73(9):2280-4. doi: 10.1002/1097-0142(19940501)73:9<2280::aid-cncr2820730908>3.0.co;2-3.

Abstract

Background: The expression of nm23-H1 and nm23-H2 proteins in 25 hepatocellular carcinomas was studied immunohistochemically.

Methods: Tissue specimens were reacted with anti-human nm23-H1 and nm23-H2 monoclonal antibodies (MoAb) (H1-229 and H2-206, respectively) and then stained by the biotin-streptoavidin complex method.

Results: Adjacent nontumorous tissues were intensely stained with nm23-H1 and nm23-H2. Of the 25 hepatocellular carcinomas, 60% were positive for MoAb H1-229, and 68% were positive for MoAb H2-206. These immunoreactivities were most common in the cytoplasm of tumor cells. There was no significant correlation between the expression of nm23-H1 protein and tumor size, Edmondson's histopathologic classification, or invasion of the capsule. However, the authors observed an inverse relationship between nm23-H1 expression and intrahepatic metastases of hepatocellular carcinomas. There was no significant correlation between the expression of nm23-H2 protein and clinicopathologic findings. Only a short survival period was observed in patients with hepatocellular carcinoma with reduced nm23-H1 or nm23-H2 proteins.

Conclusions: The results suggest that nm23-H1 protein plays a role in the suppression of intrahepatic metastasis of hepatocellular carcinoma and that the combined expression of nm23-H1 is associated with favorable prognosis.

MeSH terms

  • Carcinoma, Hepatocellular / genetics*
  • Carcinoma, Hepatocellular / pathology
  • Carcinoma, Hepatocellular / secondary
  • Cytoplasm / ultrastructure
  • Gene Expression Regulation, Neoplastic*
  • Hepatitis B Surface Antigens / blood
  • Humans
  • Immunohistochemistry
  • Liver / chemistry
  • Liver / pathology
  • Liver Cirrhosis / pathology
  • Liver Neoplasms / genetics*
  • Liver Neoplasms / pathology
  • Monomeric GTP-Binding Proteins*
  • NM23 Nucleoside Diphosphate Kinases
  • Neoplasm Invasiveness
  • Neoplasm Staging
  • Nucleoside-Diphosphate Kinase / analysis
  • Nucleoside-Diphosphate Kinase / genetics*
  • Prognosis
  • Survival Rate
  • Transcription Factors / analysis
  • Transcription Factors / genetics*

Substances

  • Hepatitis B Surface Antigens
  • NM23 Nucleoside Diphosphate Kinases
  • Transcription Factors
  • NME1 protein, human
  • Nucleoside-Diphosphate Kinase
  • Monomeric GTP-Binding Proteins