res2+, a new member of the cdc10+/SWI4 family, controls the 'start' of mitotic and meiotic cycles in fission yeast

EMBO J. 1994 Apr 15;13(8):1873-80.

Abstract

In the fission yeast Schizosaccharomyces pombe, the cdc10+ and res1+ genes play a crucial role in the start of mitotic and meiotic cycles. They encode structurally related transcriptional complex proteins and regulate some S phase-specific genes. Here we report the identification of a new member of this family named as res2+. res2+ has been isolated as a multicopy suppressor of a res1- null mutant and specifies a 73 kDa protein, which has two copies of the Swi/ankyrin motif and shares the highest sequence and structure similarity with the Res1 protein. res2+ is largely redundant in function with res1+ and is required for the initiation of mitotic and premeiotic DNA synthesis, but has an additional role in meiotic division. Unlike res1+, res2+ is highly induced during conjugation and strongly depends on cdc10+ for its activity. We conclude that the fission yeast contains two functionally overlapping parallel 'start' systems, Res1-Cdc10 and Res2-Cdc10, the former of which plays a major role in mitotic cycle whereas the latter in meiotic cycle.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Cycle Proteins*
  • Conjugation, Genetic
  • DNA-Binding Proteins*
  • Fungal Proteins / genetics*
  • GTP Phosphohydrolases
  • Genes, Fungal / genetics*
  • Meiosis / genetics*
  • Membrane Proteins
  • Mitosis / genetics*
  • Molecular Sequence Data
  • Multigene Family
  • Saccharomyces cerevisiae Proteins*
  • Schizosaccharomyces / genetics
  • Schizosaccharomyces / growth & development*
  • Schizosaccharomyces pombe Proteins*
  • Sequence Homology, Amino Acid
  • Suppression, Genetic
  • Transcription Factors*

Substances

  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • Fungal Proteins
  • Membrane Proteins
  • SWI4 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins
  • Transcription Factors
  • cdc10 protein, S pombe
  • res1 protein, S pombe
  • res2 protein, S pombe
  • CDC10 protein, S cerevisiae
  • GTP Phosphohydrolases

Associated data

  • GENBANK/D17761