Incidence of chromosome 18 disomy in human sperm nuclei as detected by nonisotopic in situ hybridization

Hum Genet. 1994 Apr;93(4):421-3. doi: 10.1007/BF00201667.


Nonradioactive in situ hybridization with an alpha-satellite DNA probe specific for chromosome 18 was performed on human interphase sperm nuclei to detect the frequency of sperm cells disomic for chromosome 18. A total of 16,127 sperm heads from eight healthy donors, aged 23-57 years, was investigated, and a minimum of 2000 sperm nuclei per proband was analyzed. The disomy rate ranged from 0.25% to 0.5%, with an average of 0.36%. This frequency does not differ significantly from that determined for other chromosomes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Cell Nucleus
  • Chromosomes, Human, Pair 18*
  • DNA Probes
  • Humans
  • In Situ Hybridization / methods*
  • Isotopes
  • Male
  • Middle Aged
  • Sperm Head
  • Spermatozoa / ultrastructure*


  • DNA Probes
  • Isotopes