Guanine nucleotide-dependent assembly of FtsZ into filaments

J Bacteriol. 1994 May;176(9):2754-8. doi: 10.1128/jb.176.9.2754-2758.1994.


FtsZ is an essential cell division protein that is localized to the leading edge of the bacterial septum in a cytokinetic ring. It contains the tubulin signature motif and is a GTP binding protein with a GTPase activity. Further comparison of FtsZ with eukaryotic tubulins revealed some additional sequence similarities, perhaps indicating a similar GTP binding site. Examination of FtsZ incubated in vitro by electron microscopy revealed a guanine nucleotide-dependent assembly into protein filaments, supporting the hypothesis that the FtsZ ring is formed through self-assembly. FtsZ3, which is unable to bind GTP, does not polymerize, whereas FtsZ2, which binds GTP but is deficient in GTP hydrolysis, is capable of polymerization.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / drug effects
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Bacterial Proteins / ultrastructure
  • Cytoskeletal Proteins*
  • Escherichia coli
  • GTP-Binding Proteins / drug effects
  • GTP-Binding Proteins / genetics
  • GTP-Binding Proteins / metabolism*
  • GTP-Binding Proteins / ultrastructure
  • Guanine Nucleotides / pharmacology*
  • Molecular Sequence Data
  • Negative Staining
  • Sequence Homology, Amino Acid
  • Tubulin / genetics


  • Bacterial Proteins
  • Cytoskeletal Proteins
  • FtsZ protein, Bacteria
  • Guanine Nucleotides
  • Tubulin
  • GTP-Binding Proteins