Quantitation of follicular non-Hodgkin's lymphoma cells carrying t(14;18) in a patient before and after allogeneic bone marrow transplantation

Ann Oncol. 1994:5 Suppl 1:43-5. doi: 10.1093/annonc/5.suppl_1.s43.

Abstract

Background: A competitive PCR method was developed, enabling accurate quantitation of residual lymphoma cells carrying the t(14;18) in blood and bone marrow samples of follicular non-Hodgkin's lymphoma (NHL) patients during treatment.

Patients and methods: A patient with residual lymphoma cells received an allogeneic bone marrow transplantation (BMT). Since BMT, the patient has been in continuing clinical complete remission.

Results: Using the competitive PCR and two-step PCR method, we were able to demonstrate a gradual decline in the number of lymphoma cells within consecutive patient blood and bone marrow samples after BMT.

Conclusions: The competitive PCR is a suitable method for the detection of minimal residual disease. Further research might reveal the clinical relevance of data obtained by this method.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Base Sequence
  • Bone Marrow Transplantation*
  • Cell Count
  • Chromosomes, Human, Pair 14*
  • Chromosomes, Human, Pair 18*
  • Humans
  • Lymphoma, Follicular / genetics*
  • Lymphoma, Follicular / pathology
  • Lymphoma, Follicular / therapy
  • Male
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Translocation, Genetic*
  • Transplantation, Homologous