Extensive intracellular translocations of a major protein accompany anoxia in embryos of Artemia franciscana

Exp Cell Res. 1994 May;212(1):77-83. doi: 10.1006/excr.1994.1120.


Cells of encysted gastrula embryos of the crustacean Artemia franciscana exhibit extraordinary stability during prolonged anoxia. We find that they contain an abundant protein (referred to as "26-kDa protein") that undergoes translocation to the nucleus during anoxia. The reverse translocation rapidly occurs when anoxic embryos are returned to aerobic conditions. A similar translocation appears to take place in embryos exposed to 42 degrees C aerobic heat shock and prolonged exposure to low temperature (0-2 degrees C), and in diapause embryos. Gel filtration and Western immunoblotting indicate that the 26-kDa protein is translocated to other cellular compartments and may also be associated with a wide variety of "soluble" proteins during anoxia. This protein makes up roughly 15% of the total nonyolk embryo protein and is, by far, most abundant in the encysted embryo stage of the life cycle. The hypothesis is advanced that the 26-kDa protein may play the role of a metabolic regulator and/or a protective molecular chaperone during prolonged anoxia and other forms of stress.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aerobiosis / physiology
  • Anaerobiosis / physiology
  • Animals
  • Artemia / embryology
  • Artemia / metabolism*
  • Biological Transport
  • Cell Compartmentation*
  • Cell Fractionation
  • Cell Nucleus / metabolism
  • Proteins / metabolism*


  • Proteins