Cloning, structure, cellular localization, and possible function of the tumor suppressor gene lethal(3)malignant blood neoplasm-1 of Drosophila melanogaster

Dev Biol. 1994 May;163(1):98-111. doi: 10.1006/dbio.1994.1126.

Abstract

The tumor suppressor gene, lethal(3)malignant blood neoplasm-1+, of Drosophila melanogaster is required for the differentiation of the phagocytic blood-cell type, the plasmatocyte. In the homozygously mutated state it causes the malignant transformation of these blood cells. We present here the cloning, sequencing, structure, and expression of the l(3)mbn-1+ gene during development. The cloned gene was identified by germ-line transformation, generation of revertants, and the detection of the corresponding mRNA in blood cells and other tissues. Homologies of the G-S-rich C-terminus of the putative MBN83 protein to human cytokeratins K1, K10, and mouse loricrin were found. The structure and possible function of the wild-type l(3)mbn-1+ gene are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blood Cells / pathology*
  • Cell Transformation, Neoplastic
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA, Complementary / analysis
  • Drosophila melanogaster / genetics*
  • Genes, Tumor Suppressor*
  • Molecular Sequence Data
  • RNA, Messenger / analysis

Substances

  • DNA, Complementary
  • RNA, Messenger

Associated data

  • GENBANK/Z47722