Repeated cleaning of protein A affinity column with sodium hydroxide

J Immunol Methods. 1994 May 2;171(1):15-21. doi: 10.1016/0022-1759(94)90223-2.

Abstract

Protein A attached to an affinity adsorbent was shown to be remarkably resistant to 0.5 M NaOH. Even repeated treatments gave only a small decrease in functional capacity and no adverse effect on leakage of the protein A into the eluate. This simple cleaning procedure should be useful in applications where antibodies need to be purified free from the risk of contamination with endotoxins or micro-organisms, e.g., for in vivo therapy, either in clinical trials or experimental animals. It can also prevent cross-contamination when the same protein A adsorbent is used for different batches of antibody.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Animals
  • Antibodies / isolation & purification
  • Antibodies, Monoclonal
  • Chromatography, Affinity / methods
  • Detergents
  • Feasibility Studies
  • Humans
  • Mice
  • Rats
  • Sodium Hydroxide*
  • Staphylococcal Protein A / chemistry
  • Staphylococcal Protein A / isolation & purification*

Substances

  • Antibodies
  • Antibodies, Monoclonal
  • Detergents
  • Staphylococcal Protein A
  • Sodium Hydroxide