BmCF1, a Bombyx mori RXR-type receptor related to the Drosophila ultraspiracle

J Mol Biol. 1994 May 6;238(3):479-86. doi: 10.1006/jmbi.1994.1306.


A PCR approach has been used to obtain an ovarian cDNA clone from the silkmoth Bombyx mori, encoding a 50 kDa protein (BmCF1) that belongs to the RXR subfamily of nuclear hormone receptors and is most similar to the CF1/USP protein (DmCF1) encoded by the ultraspiracle gene of Drosophila. The similarity is high in the DNA-binding and moderately so in the ligand-binding domains, although not in the N-terminal, putatively activator A/B domain. Protein sequence comparisons with the available RXR sequences indicate that although insect USP-like sequences are more related to each other than to vertebrate RXRs, their inter se similarities are lower than in the case of the vertebrate RXRs. Two distinct BmCF1-homologous transcripts are observed consistently, and are indicative either of alternative splicing or of the existence of a second RXR gene in the moth. The transcripts are widely distributed, suggesting functions at multiple developmental stages, as in the case of Drosophila ultraspiracle.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Bombyx / chemistry
  • Bombyx / genetics*
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics*
  • Drosophila / chemistry
  • Drosophila / genetics*
  • Drosophila Proteins
  • Female
  • Genes, Insect
  • Molecular Sequence Data
  • Ovary / metabolism
  • Polymerase Chain Reaction
  • Receptors, Cytoplasmic and Nuclear / chemistry
  • Receptors, Cytoplasmic and Nuclear / genetics*
  • Receptors, Retinoic Acid*
  • Receptors, Steroid / chemistry
  • Receptors, Steroid / genetics*
  • Retinoid X Receptors
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Transcription Factors / chemistry
  • Transcription Factors / genetics*
  • Transcription, Genetic


  • DNA, Complementary
  • DNA-Binding Proteins
  • Drosophila Proteins
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Retinoic Acid
  • Receptors, Steroid
  • Retinoid X Receptors
  • Transcription Factors
  • USP protein, Drosophila
  • BmCF1 protein, Bombyx mori

Associated data

  • GENBANK/U06073